2cdc
From Proteopedia
(New page: 200px<br /> <applet load="2cdc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cdc, resolution 1.50Å" /> '''SULFOLOBUS SOLFATAR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CDC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]] with XYS, XYP, ZN, NAP and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CDC OCA]]. | + | 2CDC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]] with XYS, XYP, ZN, NAP and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CDC OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: reductase]] | [[Category: reductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:56:59 2007'' |
Revision as of 09:52, 30 October 2007
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SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 IN COMPLEX WITH NADP AND XYLOSE
Overview
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally, above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose, and galactose. The first enzyme in this pathway, glucose dehydrogenase, catalyzes the oxidation of glucose to gluconate, but has been shown to, have activity with a broad range of sugar substrates, including glucose, galactose, xylose, and L-arabinose, with a requirement for the glucose, stereo configuration at the C2 and C3 positions. Here we report the, crystal structure of the apo form of glucose dehydrogenase to a resolution, of 1.8 A and a complex with its required cofactor, NADP+, to a resolution, of 2.3 A. A T41A mutation was engineered to enable the trapping of, substrate in the ... [(full description)]
About this Structure
2CDC is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with XYS, XYP, ZN, NAP and EDO as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus., Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL, J Biol Chem. 2006 May 26;281(21):14796-804. Epub 2006 Mar 23. PMID:16556607
Page seeded by OCA on Tue Oct 30 11:56:59 2007
Categories: Single protein | Sulfolobus solfataricus | Danson, M.J. | Hough, D.W. | Lamble, H.J. | Milburn, C.C. | Taylor, G.L. | Theodossis, A. | EDO | NAP | XYP | XYS | ZN | Glucose dehydrogenase | Mdr family | Oxidoreductase | Reductase
