2cdm
From Proteopedia
(New page: 200px<br /> <applet load="2cdm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cdm, resolution 2.70Å" /> '''THE STRUCTURE OF TR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CDM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CDM OCA]]. | + | 2CDM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CDM OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: relaxase]] | [[Category: relaxase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:57:04 2007'' |
Revision as of 09:52, 30 October 2007
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THE STRUCTURE OF TRWC COMPLEXED WITH A 27-MER DNA COMPRISING THE RECOGNITION HAIRPIN AND THE CLEAVAGE SITE
Overview
TrwC is a DNA strand transferase that catalyzes the initial and final, stages of conjugative DNA transfer. We have solved the crystal structure, of the N-terminal relaxase domain of TrwC in complex with a 27 base-long, DNA oligonucleotide that contains both the recognition hairpin and the, scissile phosphate. In addition, a series of ternary structures of, protein-DNA complexes with different divalent cations at the active site, have been solved. Systematic anomalous difference analysis allowed us to, determine unambiguously the nature of the metal bound. Zn2+, Ni2+ and Cu2+, were found to bind the histidine-triad metal binding site. Comparison of, the structures of the different complexes suggests two pathways for the, DNA to exit the active pocket. They are probably used at different ... [(full description)]
About this Structure
2CDM is a [Protein complex] structure of sequences from [Escherichia coli] with SO4 as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Unveiling the molecular mechanism of a conjugative relaxase: The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site., Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Perez-Luque R, Coll M, de la Cruz F, J Mol Biol. 2006 May 5;358(3):857-69. Epub 2006 Feb 28. PMID:16540117
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