1zru

From Proteopedia

---

Revision as of 15:41, 29 January 2008

proteopedia linkproteopedia link

spinqualitylabelsall models 1zru, resolution 1.73Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

structure of the lactophage p2 receptor binding protein in complex with glycerol

Overview

Phage p2, a member of the lactococcal 936 phage species, infects, Lactococcus lactis strains by binding initially to specific carbohydrate, receptors using its receptor-binding protein (RBP). The structures of p2, RBP, a homotrimeric protein composed of three domains, and of its complex, with a neutralizing llama VH domain (VHH5) have been determined (S., Spinelli, A. Desmyter, C. T. Verrips, H. J. de Haard, S. Moineau, and C., Cambillau, Nat. Struct. Mol. Biol. 13:85-89, 2006). Here, we show that, VHH5 was able to neutralize 12 of 50 lactococcal phages belonging to the, 936 species. Moreover, escape phage mutants no longer neutralized by VHH5, were isolated from 11 of these phages. All of the mutations (but one), cluster in the RBP/VHH5 interaction surface that delineates the, receptor-binding area. A glycerol molecule, observed in the 1.7-A, resolution structure of RBP, was found to bind tightly (Kd= 0.26 microM), in a crevice located in this area. Other saccharides bind RBP with, comparable high affinity. These data prove the saccharidic nature of the, bacterial receptor recognized by phage p2 and identify the position of its, binding site in the RBP head domain.

About this Structure

1ZRU is a Single protein structure of sequence from Lactococcus lactis phage p475 with as ligand. Full crystallographic information is available from OCA.

Reference

Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding site., Tremblay DM, Tegoni M, Spinelli S, Campanacci V, Blangy S, Huyghe C, Desmyter A, Labrie S, Moineau S, Cambillau C, J Bacteriol. 2006 Apr;188(7):2400-10. PMID:16547026

Page seeded by OCA on Tue Jan 29 17:41:32 2008