229d

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DNA ANALOG OF YEAST TRANSFER RNA PHE ANTICODON DOMAIN WITH MODIFIED BASES 5-METHYL CYTOSINE AND 1-METHYL GUANINE

Overview

Design of biologically active DNA analogues of the yeast tRNA(Phe), anticodon domain, tDNAPheAC, required the introduction of a, d(m5C)-dependent, Mg(2+)-induced structural transition and the d(m1G), disruption of an intra-loop dC.dG base pair. The modifications were, introduced at residues corresponding to m5C-40 and wybutosine-37 in, tRNA(Phe). Modified tDNAPheAC inhibited translation by 50% at a, tDNAPheAC:ribosome ratio of 8:1. The molecule's structure has been, determined by NMR spectroscopy and restrained molecular dynamics with an, overall r.m.s.d. of 2.8 A and 1.7 A in the stem, and is similar to the, tRNA(Phe) anticodon domain in conformation and dimensions. The tDNAPheAC, structure may provide a guide for the design of translation inhibitors as, potential therapeutic agents.

About this Structure

229D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:8548453

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