1gxs
From Proteopedia
(New page: 200px<br /> <applet load="1gxs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gxs, resolution 2.30Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GXS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]] with NAG, BEZ and DKA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.11 4.1.2.11]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GXS OCA]]. | + | 1GXS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]] with NAG, BEZ and DKA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydroxymandelonitrile_lyase Hydroxymandelonitrile lyase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.11 4.1.2.11]]. Structure known Active Site: CA1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GXS OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme., Lauble H, Miehlich B, Forster S, Wajant H, Effenberger F, Biochemistry. 2002 Oct 8;41(40):12043-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12356304 12356304] | Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme., Lauble H, Miehlich B, Forster S, Wajant H, Effenberger F, Biochemistry. 2002 Oct 8;41(40):12043-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12356304 12356304] | ||
| + | [[Category: Hydroxymandelonitrile lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sorghum bicolor]] | [[Category: Sorghum bicolor]] | ||
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[[Category: inhibitor complex]] | [[Category: inhibitor complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:58:26 2007'' |
Revision as of 09:53, 30 October 2007
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CRYSTAL STRUCTURE OF HYDROXYNITRILE LYASE FROM SORGHUM BICOLOR IN COMPLEX WITH INHIBITOR BENZOIC ACID: A NOVEL CYANOGENIC ENZYME
Overview
The crystal structure of the hydroxynitrile lyase from Sorghum bicolor, (SbHNL) in complex with the inhibitor benzoic acid has been determined at, 2.3 A resolution and refined to a crystallographic R-factor of 16.5%. The, SbHNL sequence places the enzyme in the alpha/beta hydrolase family where, the active site nucleophile is predicted to be organized in a, characteristic pentapeptide motif which is part of the active site, strand-turn-helix motif. In SbHNL, however, a unique two-amino acid, deletion is next to the putative active site Ser158, removing thereby the, putative oxyanion hole-forming Tyr residue. The presented X-ray structure, shows that the overall folding pattern of SbHNL is similar to that of the, closely related wheat serine carboxypeptidase (CPD-WII); however, the, ... [(full description)]
About this Structure
1GXS is a [Single protein] structure of sequence from [Sorghum bicolor] with NAG, BEZ and DKA as [ligands]. Active as [Hydroxymandelonitrile lyase], with EC number [4.1.2.11]. Structure known Active Site: CA1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme., Lauble H, Miehlich B, Forster S, Wajant H, Effenberger F, Biochemistry. 2002 Oct 8;41(40):12043-50. PMID:12356304
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