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237d
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(New page: 200px<br /><applet load="237d" size="350" color="white" frame="true" align="right" spinBox="true" caption="237d, resolution 2.500Å" /> '''CRYSTAL STRUCTURE O...)
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Revision as of 15:48, 29 January 2008
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CRYSTAL STRUCTURE OF A DNA DECAMER SHOWING A NOVEL PSEUDO FOUR-WAY HELIX-HELIX JUNCTION
Overview
The crystal structure of the decanucleotide d(CGCAATTGCG)2 has been solved, by a combination of molecular replacement and heavy-atom procedures and, has been refined to an R factor of 20.2% at 2.7 A. It is not a fully, base-paired duplex but has a central core of eight Watson-Crick base pairs, flanked by unpaired terminal guanosines and cytosines. These participate, in hydrogen-bonding arrangements with adjacent decamer duplexes in the, crystal lattice. The unpaired guanosines are bound in the G+C regions of, duplex minor grooves. The cytosines have relatively high mobility, even, though they are constrained to be in one region where they are involved in, base-paired triplets with G.C base pairs. The 5'-AATT sequence in the, duplex region has a narrow minor groove, providing further confirmation of, the sequence-dependent nature of groove width.
About this Structure
237D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DNA decamer showing a novel pseudo four-way helix-helix junction., Spink N, Nunn CM, Vojtechovsky J, Berman HM, Neidle S, Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10767-71. PMID:7479880
Page seeded by OCA on Tue Jan 29 17:48:30 2008
