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243d
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(New page: 200px<br /><applet load="243d" size="350" color="white" frame="true" align="right" spinBox="true" caption="243d, resolution 1.900Å" /> '''STRUCTURE OF THE DN...)
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Revision as of 15:48, 29 January 2008
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STRUCTURE OF THE DNA OCTANUCLEOTIDE D(ACGTACGT)2
Overview
d(ACGTACGT), C(78)H(84)N(30)O(32)P(7).20H(2)O, M(r) (DNA) = 2170, tetragonal, P4(3)2(1)2 (No 96), a = 42.845 (1), b = 42.845(1), c = 24.804, (1) A, V = 45532.5 (2) A(3), z = 8,lambda(MoKalpha) = 0.71069 A, micro, (MoKalpha) = 0.10 mm(-1), T = 295 K, R = 0.18 for 1994 unique reflections, between 5.0 and 1.9 A resolution. The self-complementary octanucleotide, d(ACGTACGT)(2) has been crystallized and its structure determined to a, resolution of 1.9 A. The asymmetric unit consists of a single strand of, octamer with 20 water molecules. It is only the second example of an, octanucleotide having terminal A.T base pairs whose structure has been, determined by X-ray crystallography. The sequence adopts the modified, A-type conformation found for all octanucleotide duplexes studied to date, with the helix bent by approximately 15 degrees and an average tilt angle, of 0 degrees. Unusually the data collection was carried out using a 3 kW, molybdenum sealed-tube source. The conformational details are discussed in, comparison with other closely related sequences.
About this Structure
243D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the DNA octanucleotide d(ACGTACGT)2., Wilcock DJ, Adams A, Cardin CJ, Wakelin LP, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):481-5. PMID:15299669
Page seeded by OCA on Tue Jan 29 17:48:45 2008
