2a43
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="2a43" size="350" color="white" frame="true" align="right" spinBox="true" caption="2a43, resolution 1.34Å" /> '''Crystal Structure of...)
Next diff →
Revision as of 15:54, 29 January 2008
|
Crystal Structure of a Luteoviral RNA Pseudoknot and Model for a Minimal Ribosomal Frameshifting Motif
Overview
To understand the role of structural elements of RNA pseudoknots in, controlling the extent of -1-type ribosomal frameshifting, we determined, the crystal structure of a high-efficiency frameshifting mutant of the, pseudoknot from potato leaf roll virus (PLRV). Correlations of the, structure with available in vitro frameshifting data for PLRV pseudoknot, mutants implicate sequence and length of a stem-loop linker as modulators, of frameshifting efficiency. Although the sequences and overall structures, of the RNA pseudoknots from PLRV and beet western yellow virus (BWYV) are, similar, nucleotide deletions in the linker and adjacent minor groove loop, abolish frameshifting only with the latter. Conversely, mutant PLRV, pseudoknots with up to four nucleotides deleted in this region exhibit, nearly wild-type frameshifting efficiencies. The crystal structure helps, rationalize the different tolerances for deletions in the PLRV and BWYV, RNAs, and we have used it to build a three-dimensional model of the PRLV, pseudoknot with a four-nucleotide deletion. The resulting structure, defines a minimal RNA pseudoknot motif composed of 22 nucleotides capable, of stimulating -1-type ribosomal frameshifts.
About this Structure
2A43 is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a luteoviral RNA pseudoknot and model for a minimal ribosomal frameshifting motif., Pallan PS, Marshall WS, Harp J, Jewett FC 3rd, Wawrzak Z, Brown BA 2nd, Rich A, Egli M, Biochemistry. 2005 Aug 30;44(34):11315-22. PMID:16114868
Page seeded by OCA on Tue Jan 29 17:54:02 2008
