2a51

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Structure of the (13-51) domain of the nucleocapsid protein NCp8 from SIVlhoest

Overview

The NCps (nucleocapsid proteins) of HIV-1 (HIV type 1), HIV-2 and SIV, (simian immunodeficiency virus) are small highly basic proteins, characterized by the presence of two CCHC ZF (zinc finger) domains. NCps, closely associated with the dimeric RNA genome in the core of the virus, particle, were shown to promote the specific encapsidation of the viral, RNA and are implicated in reverse transcription. Solution structure of the, HIV-1 NCp7 and complexes of NCp7 with RNA or DNA showed the critical, relationships between the structure and its various functions. HIV-1 and, HIV-2 have resulted respectively from transmissions of SIV from, chimpanzees and sooty mangabeys. It has been shown that the SIVlhoest (SIV, from l'Hoest monkeys) also has the potential to infect human populations., Since monkeys are of great interest for clinical studies of antiviral, drugs, the structure of (13-51)NCp8 (zinc finger domain of NCp8, encompassing residues 13-51) from SIVlhoest was determined by NMR to, appraise the influence of major differences in the sequence, since Glu21, Gly43 and Met46 in NCp7 are replaced by Pro, Glu and Phe respectively in, this particular NCp8. The structure of (13-51)NCp8 is very well defined, and surprisingly the structure of each ZF is similar in NCp7 and NCp8., Moreover, contrary to NCp7, the two ZFs are strongly locked to each other, in this NCp8. This first reported structure of a simian NCp8 compared with, that of NCp7 shows that the main structural differences occur at the, flexible linker between the two ZFs but the essential residues responsible, for the interaction with oligonucleotides adopt the same orientation in, the two proteins.

About this Structure

2A51 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Structure of the zinc finger domain encompassing residues 13-51 of the nucleocapsid protein from simian immunodeficiency virus., Morellet N, Meudal H, Bouaziz S, Roques BP, Biochem J. 2006 Feb 1;393(Pt 3):725-32. PMID:16229684

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