1cp9
From Proteopedia
(New page: 200px<br /> <applet load="1cp9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cp9, resolution 2.50Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1CP9 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Providencia_rettgeri Providencia rettgeri]] with CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CP9 OCA]]. | + | 1CP9 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Providencia_rettgeri Providencia rettgeri]] with CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]]. Structure known Active Site: AS. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CP9 OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri., McDonough MA, Klei HE, Kelly JA, Protein Sci. 1999 Oct;8(10):1971-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10548042 10548042] | Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri., McDonough MA, Klei HE, Kelly JA, Protein Sci. 1999 Oct;8(10):1971-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10548042 10548042] | ||
| + | [[Category: Penicillin amidase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Providencia rettgeri]] | [[Category: Providencia rettgeri]] | ||
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[[Category: penicillin binding protein]] | [[Category: penicillin binding protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:59:39 2007'' |
Revision as of 09:54, 30 October 2007
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CRYSTAL STRUCTURE OF PENICILLIN G ACYLASE FROM THE BRO1 MUTANT STRAIN OF PROVIDENCIA RETTGERI
Overview
Penicillin G acylase is an important enzyme in the commercial production, of semisynthetic penicillins used to combat bacterial infections. Mutant, strains of Providencia rettgeri were generated from wild-type cultures, subjected to nutritional selective pressure. One such mutant, Bro1, was, able to use 6-bromohexanamide as its sole nitrogen source. Penicillin, acylase from the Bro1 strain exhibited an altered substrate specificity, consistent with the ability of the mutant to process 6-bromohexanamide., The X-ray structure determination of this enzyme was undertaken to, understand its altered specificity and to help in the design of, site-directed mutants with desired specificities. In this paper, the, structure of the Bro1 penicillin G acylase has been solved at 2.5 A, resolution by ... [(full description)]
About this Structure
1CP9 is a [Protein complex] structure of sequences from [Providencia rettgeri] with CA and SO4 as [ligands]. Active as [Penicillin amidase], with EC number [3.5.1.11]. Structure known Active Site: AS. Full crystallographic information is available from [OCA].
Reference
Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri., McDonough MA, Klei HE, Kelly JA, Protein Sci. 1999 Oct;8(10):1971-81. PMID:10548042
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