This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5cp4
From Proteopedia
(New page: 200px<br /> <applet load="5cp4" size="450" color="white" frame="true" align="right" spinBox="true" caption="5cp4, resolution 1.75Å" /> '''CRYOGENIC STRUCTURE...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 5CP4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with K, HEM, CAM and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5CP4 OCA]]. | + | 5CP4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with K, HEM, CAM and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Structure known Active Site: K. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5CP4 OCA]]. |
==Reference== | ==Reference== | ||
Understanding the role of the essential Asp251 in cytochrome p450cam using site-directed mutagenesis, crystallography, and kinetic solvent isotope effect., Vidakovic M, Sligar SG, Li H, Poulos TL, Biochemistry. 1998 Jun 30;37(26):9211-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9649301 9649301] | Understanding the role of the essential Asp251 in cytochrome p450cam using site-directed mutagenesis, crystallography, and kinetic solvent isotope effect., Vidakovic M, Sligar SG, Li H, Poulos TL, Biochemistry. 1998 Jun 30;37(26):9211-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9649301 9649301] | ||
| + | [[Category: Camphor 5-monooxygenase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 27: | ||
[[Category: p450]] | [[Category: p450]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:59:52 2007'' |
Revision as of 09:55, 30 October 2007
|
CRYOGENIC STRUCTURE OF P450CAM
Overview
Proton transfer in cytochromes P450 is a critical step in the activation, of molecular oxygen. Extensive study of the P450cam active site has, identified several residues that play a central role in dioxygen bond, scission. A highly conserved carboxylate, aspartate-251 in P450cam in the, distal helix I, participates in a series of hydrogen-bond/ion pairs near, the molecular surface and has been implicated in the catalytic mechanism., Mutation of Asp251 is known to lower activity by 2 orders of magnitude and, change the rate-limiting step in the catalytic cycle, suggesting a role, for an acid functionality in generation of iron-oxygen reactive, intermediates. The turnover rates of the Asp251Asn mutant in various, protium-deuterium mixtures have been determined and show a significantly, ... [(full description)]
About this Structure
5CP4 is a [Single protein] structure of sequence from [Pseudomonas putida] with K, HEM, CAM and GOL as [ligands]. Active as [Camphor 5-monooxygenase], with EC number [1.14.15.1]. Structure known Active Site: K. Full crystallographic information is available from [OCA].
Reference
Understanding the role of the essential Asp251 in cytochrome p450cam using site-directed mutagenesis, crystallography, and kinetic solvent isotope effect., Vidakovic M, Sligar SG, Li H, Poulos TL, Biochemistry. 1998 Jun 30;37(26):9211-9. PMID:9649301
Page seeded by OCA on Tue Oct 30 11:59:52 2007
Categories: Camphor 5-monooxygenase | Pseudomonas putida | Single protein | Li, H. | Poulos, T.L. | CAM | GOL | HEM | K | Electron transport | Heme enzyme | Monooxygenase | Oxidoreductase | P450
