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2aqj

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(New page: 200px<br /><applet load="2aqj" size="350" color="white" frame="true" align="right" spinBox="true" caption="2aqj, resolution 1.800&Aring;" /> '''The structure of tr...)
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Revision as of 16:07, 29 January 2008

Image:2aqj.gif

2aqj, resolution 1.800Å

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The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination

Overview

Chlorinated natural products include vancomycin and cryptophycin A. Their, biosynthesis involves regioselective chlorination by flavin-dependent, halogenases. We report the structural characterization of tryptophan, 7-halogenase (PrnA), which regioselectively chlorinates tryptophan., Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10, angstrom-long tunnel and bound by distinct enzyme modules. The FAD module, is conserved in halogenases and is related to flavin-dependent, monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2, to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is, guided through the tunnel to tryptophan, where it is activated to, participate in electrophilic aromatic substitution.

About this Structure

2AQJ is a Single protein structure of sequence from Pseudomonas fluorescens with , and as ligands. Full crystallographic information is available from OCA.

Reference

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462

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