User:David Jung/BCHM3981 RTP Tus
From Proteopedia
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Langly, D.B., Smith, M.T., Lewis, P.J., Wake, R.G. (1993). Protein-nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator. ''Molecular Microbiology.'' '''10'''(4):771-779. | Langly, D.B., Smith, M.T., Lewis, P.J., Wake, R.G. (1993). Protein-nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator. ''Molecular Microbiology.'' '''10'''(4):771-779. | ||
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| + | Duggin, I.G., Matthews, J.M., Dixon, N.E., Wake, R.G., Mackay, J.P. (2005). A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis. ''J. Biol. Chem.'' '''280'''(13):13105-13113. | ||
Revision as of 00:53, 13 May 2011
RTP Tus test
Contents |
History
Differential binding affinity model For each terminator site (Ter site), two dimers of RTP bind. Each dimer binds to each half site present in a terminator site. It was hypothesised that the RTP dimer binding to the half site located in the "blocking" site binds tightly while the dimer binding to the "permissive" site binds less tightly to the site. However, after a series of experiments using mutant forms of terminator sites that contain RTP binding half sites with differential binding affinity, it was concluded that this differential binding affinity model cannot solely explain the polarity of termination.
Structure
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Function
References
Langly, D.B., Smith, M.T., Lewis, P.J., Wake, R.G. (1993). Protein-nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator. Molecular Microbiology. 10(4):771-779.
Duggin, I.G., Matthews, J.M., Dixon, N.E., Wake, R.G., Mackay, J.P. (2005). A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis. J. Biol. Chem. 280(13):13105-13113.
