2auv
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(New page: 200px<br /><applet load="2auv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2auv" /> '''Solution Structure of HndAc : A Thioredoxin-...)
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Revision as of 16:10, 29 January 2008
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Solution Structure of HndAc : A Thioredoxin-like [2Fe-2S] Ferredoxin Involved in the NADP-reducing Hydrogenase Complex
Overview
The NADP-reducing hydrogenase complex from Desulfovibrio fructosovorans is, a heterotetramer encoded by the hndABCD operon. Sequence analysis, indicates that the HndC subunit (52 kDa) corresponds to the NADP-reducing, unit, and the HndD subunit (63.5 kDa) is homologous to Clostridium, pasteurianum hydrogenase. The role of HndA and HndB subunits (18.8 kDa and, 13.8 kDa, respectively) in the complex remains unknown. The HndA subunit, belongs to the [2Fe-2S] ferredoxin family typified by C. pasteurianum, ferredoxin. HndA is organized into two independent structural domains, and, we report in the present work the NMR structure of its C-terminal domain, HndAc. HndAc has a thioredoxin-like fold consisting in four beta-strands, and two relatively long helices. The [2Fe-2S] cluster is located near the, surface of the protein and bound to four cysteine residues particularly, well conserved in this class of proteins. Electron exchange between the, HndD N-terminal [2Fe-2S] domain (HndDN) and HndAc has been previously, evidenced, and in the present studies we have mapped the binding site of, the HndDN domain on HndAc. A structural analysis of HndB indicates that it, is a FeS subunit with 41% similarity with HndAc and it contains a possible, thioredoxin-like fold. Our data let us propose that HndAc and HndB can, form a heterodimeric intermediate in the electron transfer between the, hydrogenase (HndD) active site and the NADP reduction site in HndC.
About this Structure
2AUV is a Single protein structure of sequence from Desulfovibrio fructosovorans with as ligand. Active as Hydrogen dehydrogenase (NADP(+)), with EC number 1.12.1.3 Full crystallographic information is available from OCA.
Reference
Solution structure of HndAc: a thioredoxin-like domain involved in the NADP-reducing hydrogenase complex., Nouailler M, Morelli X, Bornet O, Chetrit B, Dermoun Z, Guerlesquin F, Protein Sci. 2006 Jun;15(6):1369-78. PMID:16731971
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