1gmw
From Proteopedia
(New page: 200px<br /> <applet load="1gmw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gmw, resolution 1.5Å" /> '''STRUCTURE OF UREE'''...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GMW is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GMW OCA]]. | + | 1GMW is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GMW OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: metallochaperone]] | [[Category: metallochaperone]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:01:58 2007'' |
Revision as of 09:57, 30 October 2007
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STRUCTURE OF UREE
Overview
UreE is proposed to be a metallochaperone that delivers nickel ions to, urease during activation of this bacterial virulence factor. Wild-type, Klebsiella aerogenes UreE binds approximately six nickel ions per, homodimer, whereas H144*UreE (a functional C-terminal truncated variant), was previously reported to bind two. We determined the structure of, H144*UreE by multi-wavelength anomalous diffraction and refined it to 1.5, A resolution. The present structure reveals an Hsp40-like peptide-binding, domain, an Atx1-like metal-binding domain, and a flexible C terminus., Three metal-binding sites per dimer, defined by structural analysis of, Cu-H144*UreE, are on the opposite face of the Atx1-like domain than, observed in the copper metallochaperone. One metal bridges the two, subunits via ... [(full description)]
About this Structure
1GMW is a [Protein complex] structure of sequences from [Klebsiella aerogenes] with CU as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation., Song HK, Mulrooney SB, Huber R, Hausinger RP, J Biol Chem. 2001 Dec 28;276(52):49359-64. Epub 2001 Oct 8. PMID:11591723
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