2uy8
From Proteopedia
(New page: 200px<br /> <applet load="2uy8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2uy8, resolution 2.800Å" /> '''R92A MUTANT OF BAC...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2UY8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with MN and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.2 4.1.1.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2UY8 OCA]]. | + | 2UY8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with MN and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Oxalate_decarboxylase Oxalate decarboxylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.2 4.1.1.2]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2UY8 OCA]]. |
==Reference== | ==Reference== | ||
The identity of the active site of oxalate decarboxylase and the importance of the stability of active site lid conformations., Just VJ, Burrell MR, Bowater L, McRobbie I, Stevenson CE, Lawson DM, Bornemann S, Biochem J. 2007 Aug 6;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17680775 17680775] | The identity of the active site of oxalate decarboxylase and the importance of the stability of active site lid conformations., Just VJ, Burrell MR, Bowater L, McRobbie I, Stevenson CE, Lawson DM, Bornemann S, Biochem J. 2007 Aug 6;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17680775 17680775] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| + | [[Category: Oxalate decarboxylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bornemann, S.]] | [[Category: Bornemann, S.]] | ||
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[[Category: r92a mutant]] | [[Category: r92a mutant]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:03:03 2007'' |
Revision as of 09:58, 30 October 2007
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R92A MUTANT OF BACILLUS SUBTILIS OXALATE DECARBOXYLASE OXDC
Overview
Oxalate decarboxylase (EC 4.1.1.2) catalyses the conversion of oxalate to, carbon dioxide and formate. It requires manganese and, uniquely, dioxygen, for catalysis. It forms a homohexamer and each subunit contains two, similar, but distinct, manganese sites termed sites 1 and 2. There is, kinetic evidence that only site 1 is catalytically active and that site 2, is purely structural. However, the kinetics of enzymes with mutations in, site 2 are often ambiguous and all mutant kinetics have been interpreted, without structural information. Nine new site-directed mutants have been, generated and four mutant crystal structures have now been solved. Most, mutants targeted either the flexibility (T165P), favoured conformation, (S161A, S164A, D297A or H299A) or presence (Delta162-3 or ... [(full description)]
About this Structure
2UY8 is a [Single protein] structure of sequence from [Bacillus subtilis] with MN and TRS as [ligands]. Active as [Oxalate decarboxylase], with EC number [4.1.1.2]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The identity of the active site of oxalate decarboxylase and the importance of the stability of active site lid conformations., Just VJ, Burrell MR, Bowater L, McRobbie I, Stevenson CE, Lawson DM, Bornemann S, Biochem J. 2007 Aug 6;. PMID:17680775
Page seeded by OCA on Tue Oct 30 12:03:03 2007
Categories: Bacillus subtilis | Oxalate decarboxylase | Single protein | Bornemann, S. | Bowater, L. | Burrell, M.R. | Just, V.J. | Lawson, D.M. | Mcrobbie, I. | Stevenson, C.E.M. | MN | TRS | Cupin | Decarboxylase | Formate | Lyase | Manganese | Metal binding protein | Metal-binding | Oxalate | R92a mutant
