2c9j
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(New page: 200px<br /><applet load="2c9j" size="350" color="white" frame="true" align="right" spinBox="true" caption="2c9j, resolution 1.35Å" /> '''STRUCTURE OF THE FLU...)
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Revision as of 16:39, 29 January 2008
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STRUCTURE OF THE FLUORESCENT PROTEIN CMFP512 AT 1.35A FROM CERIANTHUS MEMBRANACEUS
Overview
Autofluorescent proteins of the GFP family all share the same, three-dimensional beta-can fold; yet they exhibit widely different optical, properties, arising either from chemical modification of the chromophore, itself or from specific interactions of the chromophore with the, surrounding protein moiety. Here we present a structural and spectroscopic, characterization of the green fluorescent protein cmFP512 from Cerianthus, membranaceus, a nonbioluminescent, azooxanthellate cnidarian, which has, only approximately 22% sequence identity with Aequorea victoria GFP. The, X-ray structure, obtained by molecular replacement at a resolution of 1., 35 A, shows the chromophore, formed from the tripeptide Gln-Tyr-Gly, in a, hydrogen-bonded cage in the center of an 11-stranded beta-barrel, tightly, restrained by adjacent residues and structural water molecules. It exists, in a neutral (A) and an anionic (B) species, with absorption/emission, maxima at 392/460 (pH 5) and 503/512 nm (pH 7). Their fractional, populations and peak positions depend sensitively on pH, reflecting, protonation of groups adjacent to the chromophore. The pH dependence of, the spectra is explained by a protonation mechanism involving a, hydrogen-bonded cluster of charged/polar groups. Cryospectroscopy at 12 K, was also performed to analyze the vibronic coupling of the electronic, transitions.
About this Structure
2C9J is a Single protein structure of sequence from Cerianthus membranaceus. Full crystallographic information is available from OCA.
Reference
Exploring chromophore--protein interactions in fluorescent protein cmFP512 from Cerianthus membranaceus: X-ray structure analysis and optical spectroscopy., Nienhaus K, Renzi F, Vallone B, Wiedenmann J, Nienhaus GU, Biochemistry. 2006 Oct 31;45(43):12942-53. PMID:17059211
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