2cl2

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spinqualitylabelsall models 2cl2, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ENDO-1,3(4)-BETA-GLUCANASE FROM PHANEROCHAETE CHRYSOSPORIUM, SOLVED USING NATIVE SULFUR SAD, EXHIBITING INTACT HEPTASACCHARIDE GLYCOSYLATION

Overview

Laminarinase Lam16A from Phanerochaete chrysosporium was recombinantly, expressed in Pichia pastoris, crystallized and the structure was solved at, 1.34 A resolution using native sulfur SAD X-ray crystallography. It is the, first structure of a non-specific 1,3(4)-beta-D-glucanase from glycoside, hydrolase family 16 (GH16). P. chrysosporium is a wood-degrading, basidiomycete fungus and Lam16A is the predominant extracellular protein, expressed when laminarin is used as the sole carbon source. The protein, folds into a curved beta-sandwich homologous to those of other known GH16, enzyme structures (especially kappa-carrageenase from Pseudoalteromonas, carrageenovora and beta-agarase from Zobelia galactanivorans). A notable, likeness is also evident with the related glycoside hydrolase family 7, (GH7) enzymes. A mammalian lectin, p58/ERGIC, as well as polysaccharide, lyase (PL7) enzymes also showed significant similarity to Lam16A. The, enzyme has two potential N-glycosylation sites. One such site, at Asn43, displayed a branched heptasaccharide sufficiently stabilized to be, interpreted from the X-ray diffraction data. The other N-glycosylation, motif was found close to the catalytic centre and is evidently not, glycosylated.

About this Structure

2CL2 is a Single protein structure of sequence from Phanerochaete chrysosporium. Active as Endo-1,3(4)-beta-glucanase, with EC number 3.2.1.6 Full crystallographic information is available from OCA.

Reference

X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium., Vasur J, Kawai R, Larsson AM, Igarashi K, Sandgren M, Samejima M, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1422-9. Epub 2006, Oct 18. PMID:17057348

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