2bmu
From Proteopedia
(New page: 200px<br /> <applet load="2bmu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bmu, resolution 2.55Å" /> '''UMP KINASE FROM PYR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BMU is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with MG, ANP and U5P as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BMU OCA]]. | + | 2BMU is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with MG, ANP and U5P as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BMU OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: ump kinase]] | [[Category: ump kinase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:06:15 2007'' |
Revision as of 10:01, 30 October 2007
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UMP KINASE FROM PYROCOCCUS FURIOSUS COMPLEXED WITH ITS SUBSTRATE UMP AND ITS SUBSTRATE ANALOG AMPPNP
Overview
UMP kinase (UMPK), the enzyme responsible for microbial UMP, phosphorylation, plays a key role in pyrimidine nucleotide biosynthesis, regulating this process via feed-back control and via gene repression of, carbamoyl phosphate synthetase (the first enzyme of the pyrimidine, biosynthesis pathway). We present crystal structures of Pyrococcus, furiosus UMPK, free or complexed with AMPPNP or AMPPNP and UMP, at 2.4 A, 3 A and 2.55 A resolution, respectively, providing a true snapshot of the, catalytically competent bisubstrate complex. The structure proves that, UMPK does not resemble other nucleoside monophosphate kinases, including, the UMP/CMP kinase found in animals, and thus UMPK may be a potential, antimicrobial target. This enzyme has a homohexameric architecture centred, around a ... [(full description)]
About this Structure
2BMU is a [Single protein] structure of sequence from [Pyrococcus furiosus] with MG, ANP and U5P as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The crystal structure of Pyrococcus furiosus UMP kinase provides insight into catalysis and regulation in microbial pyrimidine nucleotide biosynthesis., Marco-Marin C, Gil-Ortiz F, Rubio V, J Mol Biol. 2005 Sep 16;352(2):438-54. PMID:16095620
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