1w4p
From Proteopedia
(New page: 200px<br /> <applet load="1w4p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w4p, resolution 1.69Å" /> '''BINDING OF NONNATUR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1W4P is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with UM3 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W4P OCA]]. | + | 1W4P is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with UM3 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W4P OCA]]. |
==Reference== | ==Reference== | ||
Binding of non-natural 3'-nucleotides to ribonuclease A., Jenkins CL, Thiyagarajan N, Sweeney RY, Guy MP, Kelemen BR, Acharya KR, Raines RT, FEBS J. 2005 Feb;272(3):744-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15670155 15670155] | Binding of non-natural 3'-nucleotides to ribonuclease A., Jenkins CL, Thiyagarajan N, Sweeney RY, Guy MP, Kelemen BR, Acharya KR, Raines RT, FEBS J. 2005 Feb;272(3):744-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15670155 15670155] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| + | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Acharya, K.R.]] | [[Category: Acharya, K.R.]] | ||
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[[Category: rnase a]] | [[Category: rnase a]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:07:01 2007'' |
Revision as of 10:02, 30 October 2007
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BINDING OF NONNATURAL 3'-NUCLEOTIDES TO RIBONUCLEASE A
Overview
2'-Fluoro-2'-deoxyuridine 3'-phosphate (dU(F)MP) and arabinouridine, 3'-phosphate (araUMP) have non-natural furanose rings. dU(F)MP and araUMP, were prepared by chemical synthesis and found to have three- to sevenfold, higher affinity than uridine 3'-phosphate (3'-UMP) or 2'-deoxyuridine, 3'-phosphate (dUMP) for ribonuclease A (RNase A). These differences, probably arise (in part) from the phosphoryl groups of 3'-UMP, dU(F)MP, and araUMP (pK(a) = 5.9) being more anionic than that of dUMP (pK(a) =, 6.3). The three-dimensional structures of the crystalline complexes of, RNase A with dUMP, dU(F)MP and araUMP were determined at < 1.7 A, resolution by X-ray diffraction analysis. In these three structures, the, uracil nucleobases and phosphoryl groups bind to the enzyme in a nearly, ... [(full description)]
About this Structure
1W4P is a [Single protein] structure of sequence from [Bos taurus] with UM3 as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Binding of non-natural 3'-nucleotides to ribonuclease A., Jenkins CL, Thiyagarajan N, Sweeney RY, Guy MP, Kelemen BR, Acharya KR, Raines RT, FEBS J. 2005 Feb;272(3):744-55. PMID:15670155
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