1w4y

From Proteopedia

Revision as of 10:02, 30 October 2007

FERROUS HORSERADISH PEROXIDASE C1A IN COMPLEX WITH CARBON MONOXIDE

Overview

Carbon monoxide, formate, and acetate interact with horseradish peroxidase, (HRP) by binding to subsites within the active site. These ligands also, bind to catalases, but their interactions are different in the two types, of enzymes. Formate (notionally the "hydrated" form of carbon monoxide) is, oxidized to carbon dioxide by compound I in catalase, while no such, reaction is reported to occur in HRP, and the CO complex of ferrocatalase, can only be obtained indirectly. Here we describe high-resolution crystal, structures for HRP in its complexes with carbon monoxide and with formate, and compare these with the previously determined HRP-acetate structure, [Berglund, G. I., et al. (2002) Nature 417, 463-468]. A multicrystal X-ray, data collection strategy preserved the correct oxidation ... [(full description)]

About this Structure

1W4Y is a [Single protein] structure of sequence from [Armoracia rusticana] with CA, HEM and CMO as [ligands]. Active as [Peroxidase], with EC number [1.11.1.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide., Carlsson GH, Nicholls P, Svistunenko D, Berglund GI, Hajdu J, Biochemistry. 2005 Jan 18;44(2):635-42. PMID:15641789

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