2fbk
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(New page: 200px<br /><applet load="2fbk" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fbk, resolution 2.300Å" /> '''The Crystal Structu...)
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Revision as of 17:32, 29 January 2008
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The Crystal Structure of HucR from Deinococcus radiodurans
Overview
We report here the 2.3 A resolution structure of the hypothetical uricase, regulator (HucR) from Deinococcus radiodurans R1. HucR, a member of the, MarR family of DNA-binding proteins, was previously shown to repress its, own expression as well as that of a uricase, a repression that is, alleviated both in vivo and in vitro upon binding uric acid, the substrate, for uricase. As uric acid is a potent scavenger of reactive oxygen, species, and as D. radiodurans is known for its remarkable resistance to, DNA-damaging agents, these observations indicate a novel oxidative stress, response mechanism. The crystal structure of HucR in the absence of ligand, or DNA reveals a dimer in which the DNA recognition helices are, preconfigured for DNA binding. This configuration of DNA-binding domains, is achieved through an apparently stable dimer interface that, in contrast, to what is observed in other MarR homologs for which structures have been, determined, shows little conformational heterogeneity in the absence of, ligand. An additional amino-terminal segment, absent from other MarR, homologs, appears to brace the principal helix of the dimerization, interface. However, although HucR is preconfigured for DNA binding, the, presence of a stacked pair of symmetry-related histidine residues at a, central pivot point in the dimer interface suggests a mechanism for a, conformational change to attenuate DNA binding.
About this Structure
2FBK is a Single protein structure of sequence from Deinococcus radiodurans with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the transcriptional regulator HucR from Deinococcus radiodurans reveals a repressor preconfigured for DNA binding., Bordelon T, Wilkinson SP, Grove A, Newcomer ME, J Mol Biol. 2006 Jun 30;360(1):168-77. Epub 2006 May 19. PMID:16750221
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