2fct

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(New page: 200px<br /><applet load="2fct" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fct, resolution 1.60&Aring;" /> '''SyrB2 with Fe(II), c...)
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Revision as of 17:33, 29 January 2008

Image:2fct.gif

2fct, resolution 1.60Å

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SyrB2 with Fe(II), chloride, and alpha-ketoglutarate

Overview

Non-haem Fe(II)/alpha-ketoglutarate (alphaKG)-dependent enzymes harness, the reducing power of alphaKG to catalyse oxidative reactions, usually the, hydroxylation of unactivated carbons, and are involved in processes such, as natural product biosynthesis, the mammalian hypoxic response, and DNA, repair. These enzymes couple the decarboxylation of alphaKG with the, formation of a high-energy ferryl-oxo intermediate that acts as a, hydrogen-abstracting species. All previously structurally characterized, mononuclear iron enzymes contain a 2-His, 1-carboxylate motif that, coordinates the iron. The two histidines and one carboxylate, known as the, 'facial triad', form one triangular side of an octahedral iron, coordination geometry. A subclass of mononuclear iron enzymes has been, shown to catalyse halogenation reactions, rather than the more typical, hydroxylation reaction. SyrB2, a member of this subclass, is a non-haem, Fe(II)/alphaKG-dependent halogenase that catalyses the chlorination of, threonine in syringomycin E biosynthesis. Here we report the structure of, SyrB2 with both a chloride ion and alphaKG coordinated to the iron ion at, 1.6 A resolution. This structure reveals a previously unknown coordination, of iron, in which the carboxylate ligand of the facial triad is replaced, by a chloride ion.

About this Structure

2FCT is a Single protein structure of sequence from Pseudomonas syringae pv. syringae with , , and as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis., Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL, Nature. 2006 Mar 16;440(7082):368-71. PMID:16541079

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