2ffl
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(New page: 200px<br /><applet load="2ffl" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ffl, resolution 3.33Å" /> '''Crystal Structure of...)
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Revision as of 17:35, 29 January 2008
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Crystal Structure of Dicer from Giardia intestinalis
Overview
The specialized ribonuclease Dicer initiates RNA interference by cleaving, double-stranded RNA (dsRNA) substrates into small fragments about 25, nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from, the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and, RNase III domains matches the length spanned by 25 base pairs of RNA., Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves, a specified distance from the helical end.
About this Structure
2FFL is a Single protein structure of sequence from Giardia intestinalis with as ligand. Active as Ribonuclease III, with EC number 3.1.26.3 Full crystallographic information is available from OCA.
Reference
Structural basis for double-stranded RNA processing by Dicer., Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA, Science. 2006 Jan 13;311(5758):195-8. PMID:16410517
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