This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2fl1
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="2fl1" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fl1, resolution 2.4Å" /> '''Crystal structure of ...)
Next diff →
Revision as of 17:39, 29 January 2008
|
Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution
Overview
The three-dimensional structure of the red fluorescent protein (RFP), zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric, unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in, space group C222(1). The crystal structure, refined to a crystallographic, R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed, of 11 strands similar to that of the yellow fluorescent protein zYFP538., The zRFP574 chromophore, originating from the protein sequence, Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins., The bond geometry of residue 66 shows the strong tendency of the, corresponding C(alpha) atom to sp(2) hybridization as a consequence of, N-acylimine bond formation. The zRFP574 chromophore contains the 65-66, cis-peptide bond characteristic of red fluorescent proteins. The, chromophore phenolic ring adopts a cis conformation coplanar with the, imidazolinone ring. The crystallographic study has revealed an unexpected, chemical feature of the internal chromophore. A decarboxylated side chain, of the chromophore-forming residue Asp66 has been observed in the, structure. This additional post-translational modification is likely to, play a key role in the bathochromic shift of the zRFP574 spectrum.
About this Structure
2FL1 is a Single protein structure of sequence from Zoanthus sp. with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946
Page seeded by OCA on Tue Jan 29 19:39:00 2008
Categories: Single protein | Zoanthus sp. | Martynov, V. | Pletnev, S. | Pletnev, V. | Pletneva, N. | Popov, B. | Tikhonova, T. | SO4 | Beta barrel | Beta-can fold | Button polyp | Chromophore | Crystal structure | Emission maximum 574nm | Fluorescent marker | Intersubunit interface | Red fluorescent protein | Tightly packed tetramer | Zrfp574
