2fqn

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Revision as of 17:42, 29 January 2008

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spinqualitylabelsall models 2fqn, resolution 2.300Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site

Overview

The decoding A site of the small ribosomal subunit is an RNA molecular, switch, which monitors codon-anticodon interactions to guarantee, translation fidelity. We have solved the crystal structure of an RNA, fragment containing two Homo sapiens cytoplasmic A sites. Each of the two, A sites presents a different conformational state. In one state, adenines, A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair, to A1491. In the second state, adenines A1492 and A1493 form, non-Watson-Crick pairs with C1409 and G1408, respectively while A1491, bulges out. The first state of the eukaryotic A site is, thus, basically, the same as in the bacterial A site with bulging A1492 and A1493. It is, the state used for recognition of the codon/anticodon complex. On the, contrary, the second state of the H.sapiens cytoplasmic A site is, drastically different from any of those observed for the bacterial A site, without bulging A1492 and A1493.

About this Structure

2FQN is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.

Reference

Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:16452297

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