2ivh
From Proteopedia
(New page: 200px<br /> <applet load="2ivh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ivh, resolution 2.80Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2IVH is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IVH OCA]]. | + | 2IVH is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IVH OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:09:36 2007'' |
Revision as of 10:04, 30 October 2007
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CRYSTAL STRUCTURE OF THE NUCLEASE DOMAIN OF COLE7 (H545Q MUTANT) IN COMPLEX WITH AN 18-BP DUPLEX DNA
Overview
Nonspecific endonucleases hydrolyze DNA without sequence specificity but, with sequence preference, however the structural basis for cleavage, preference remains elusive. We show here that the nonspecific endonuclease, ColE7 cleaves DNA with a preference for making nicks after (at 3'O-side), thymine bases but the periplasmic nuclease Vvn cleaves DNA more evenly, with little sequence preference. The crystal structure of the 'preferred, complex' of the nuclease domain of ColE7 bound to an 18 bp DNA with a, thymine before the scissile phosphate had a more distorted DNA phosphate, backbone than the backbones in the non-preferred complexes, so that the, scissile phosphate was compositionally closer to the endonuclease active, site resulting in more efficient DNA cleavage. On the other hand, in ... [(full description)]
About this Structure
2IVH is a [Protein complex] structure of sequences from [Escherichia coli] with ZN as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural basis for sequence-dependent DNA cleavage by nonspecific endonucleases., Wang YT, Yang WJ, Li CL, Doudeva LG, Yuan HS, Nucleic Acids Res. 2007;35(2):584-94. Epub 2006 Dec 15. PMID:17175542
Page seeded by OCA on Tue Oct 30 12:09:36 2007
Categories: Escherichia coli | Protein complex | Doudeva, L.G. | Li, C.L. | Wang, Y.T. | Yang, W.J. | Yuan, H.S. | ZN | Antibiotic | Antimicrobial | Bacteriocin | Endonuclease | Hydrolase | Hydrolase/dna complex | Metal-binding | Nuclease | Plasmid | Zinc
