2g1g
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Revision as of 17:49, 29 January 2008
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Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine
Overview
Modified nucleosides play an important role in structure and function of, tRNA. We have determined the solution structure of the anticodon stem-loop, (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of, N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet, (t6A37) results in the formation of a U-turn motif and enhances stacking, interactions within the loop and stem regions (i.e. between A35 and t6A37), by bulging out U36. This conformation was not observed in a crystal, structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification, also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A, comparison of this stem-loop with our structure demonstrates different, effects of the modification depending on the loop sequence.
About this Structure
2G1G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:16738127
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