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2g5q

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(New page: 200px<br /><applet load="2g5q" size="350" color="white" frame="true" align="right" spinBox="true" caption="2g5q, resolution 2.700&Aring;" /> '''Crystal structures ...)
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Revision as of 17:52, 29 January 2008


2g5q, resolution 2.700Å

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Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.

Overview

Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the, 2-deoxystreptamine ring (ring II) by the L-haba group. In the present, study, the crystal structure of a complex between oligonucleotide, containing the bacterial ribosomal A site and amikacin has been solved at, 2.7 A resolution. Amikacin specifically binds to the A site in practically, the same way as its parent compound kanamycin. In addition, the L-haba, group interacts with the upper side of the A site through two direct, contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal, structure shows how the introduction of the L-haba group on ring II of, aminoglycoside is an effective mutation for obtaining a higher affinity to, the bacterial A site.

About this Structure

2G5Q is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:16806634

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