1e1a
From Proteopedia
(New page: 200px<br /> <applet load="1e1a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e1a, resolution 1.8Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1E1A is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E1A OCA]]. | + | 1E1A is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2]]. Structure known Active Site: DFP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E1A OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris., Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H, Structure. 2001 Jun;9(6):493-502. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11435114 11435114] | Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris., Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H, Structure. 2001 Jun;9(6):493-502. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11435114 11435114] | ||
| + | [[Category: Diisopropyl-fluorophosphatase]] | ||
[[Category: Loligo vulgaris]] | [[Category: Loligo vulgaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 26: | ||
[[Category: selenometionine]] | [[Category: selenometionine]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:10:41 2007'' |
Revision as of 10:05, 30 October 2007
|
CRYSTAL STRUCTURE OF DFPASE FROM LOLIGO VULGARIS
Overview
BACKGROUND: Phosphotriesterases (PTE) are enzymes capable of detoxifying, organophosphate-based chemical warfare agents by hydrolysis. One subclass, of these enzymes comprises the family of diisopropylfluorophosphatases, (DFPases). The DFPase reported here was originally isolated from squid, head ganglion of Loligo vulgaris and can be characterized as squid-type, DFPase. It is capable of hydrolyzing the organophosphates, diisopropylfluorophosphate, soman, sarin, tabun, and cyclosarin. RESULTS:, Crystals were grown of both the native and the selenomethionine-labeled, enzyme. The X-ray crystal structure of the DFPase from Loligo vulgaris has, been solved by MAD phasing and refined to a crystallographic R value of, 17.6% at a final resolution of 1.8 A. Using site-directed mutagenesis, we, ... [(full description)]
About this Structure
1E1A is a [Single protein] structure of sequence from [Loligo vulgaris] with CA as [ligand]. Active as [Diisopropyl-fluorophosphatase], with EC number [3.1.8.2]. Structure known Active Site: DFP. Full crystallographic information is available from [OCA].
Reference
Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris., Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H, Structure. 2001 Jun;9(6):493-502. PMID:11435114
Page seeded by OCA on Tue Oct 30 12:10:41 2007
