2gcs
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(New page: 200px<br /><applet load="2gcs" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gcs, resolution 2.10Å" /> '''Pre-cleavage state o...)
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Revision as of 17:56, 29 January 2008
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Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Overview
The glmS ribozyme is the only natural catalytic RNA known to require a, small-molecule activator for catalysis. This catalytic RNA functions as a, riboswitch, with activator-dependent RNA cleavage regulating glmS, messenger RNA expression. We report crystal structures of the glmS, ribozyme in precleavage states that are unliganded or bound to the, competitive inhibitor glucose-6-phosphate and in the postcleavage state., All structures superimpose closely, revealing a remarkably rigid RNA that, contains a preformed active and coenzyme-binding site. Unlike other, riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of, the glmS ribozyme-bound coenzyme performs general acid-base and, electrostatic catalysis.
About this Structure
2GCS is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543
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