This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2goz
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="2goz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2goz, resolution 2.20Å" /> '''The 2.2 A structure ...)
Next diff →
Revision as of 18:04, 29 January 2008
|
The 2.2 A structure of a full-length catalytically active hammerhead ribozyme
Overview
Minimal hammerhead ribozymes have been characterized extensively by static, and time-resolved crystallography as well as numerous biochemical, analyses, leading to mutually contradictory mechanistic explanations for, catalysis. We present the 2.2 A resolution crystal structure of a, full-length Schistosoma mansoni hammerhead ribozyme that permits us to, explain the structural basis for its 1000-fold catalytic enhancement. The, full-length hammerhead structure reveals how tertiary interactions, occurring remotely from the active site prime this ribozyme for catalysis., G-12 and G-8 are positioned consistent with their previously suggested, roles in acid-base catalysis, the nucleophile is aligned with a scissile, phosphate positioned proximal to the A-9 phosphate, and previously, unexplained roles of other conserved nucleotides become apparent within, the context of a distinctly new fold that nonetheless accommodates the, previous structural studies. These interactions permit us to explain the, previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner.
About this Structure
2GOZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Tertiary contacts distant from the active site prime a ribozyme for catalysis., Martick M, Scott WG, Cell. 2006 Jul 28;126(2):309-20. Epub 2006 Jul 20. PMID:16859740
Page seeded by OCA on Tue Jan 29 20:04:51 2008
