2gsj

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Revision as of 18:07, 29 January 2008

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spinqualitylabelsall models 2gsj, resolution 1.73Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity

Overview

Parkia platycephala lectin 2 was purified from Parkia platycephala, (Leguminosae, Mimosoideae) seeds by affinity chromatography and RP-HPLC., Equilibrium sedimentation and MS showed that Parkia platycephala lectin 2, is a nonglycosylated monomeric protein of molecular mass 29 407+/-15 Da, which contains six cysteine residues engaged in the formation of three, intramolecular disulfide bonds. Parkia platycephala lectin 2 agglutinated, rabbit erythrocytes, and this activity was specifically inhibited by, N-acetylglucosamine. In addition, Parkia platycephala lectin 2 hydrolyzed, beta(1-4) glycosidic bonds linking, 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. The full-length, amino acid sequence of Parkia platycephala lectin 2, determined by, N-terminal sequencing and cDNA cloning, and its three-dimensional, structure, established by X-ray crystallography at 1.75 A resolution, showed that Parkia platycephala lectin 2 is homologous to endochitinases, of the glycosyl hydrolase family 18, which share the (betaalpha)8 barrel, topology harboring the catalytic residues Asp125, Glu127, and Tyr182.

About this Structure

2GSJ is a Protein complex structure of sequences from Parkia platycephala with as ligand. Full crystallographic information is available from OCA.

Reference

cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds., Cavada BS, Moreno FB, da Rocha BA, de Azevedo WF Jr, Castellon RE, Goersch GV, Nagano CS, de Souza EP, Nascimento KS, Radis-Baptista G, Delatorre P, Leroy Y, Toyama MH, Pinto VP, Sampaio AH, Barettino D, Debray H, Calvete JJ, Sanz L, FEBS J. 2006 Sep;273(17):3962-74. PMID:16934035

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