2gv3
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(New page: 200px<br /><applet load="2gv3" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gv3" /> '''Translocation of a tRNA with an extended ant...)
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Revision as of 18:08, 29 January 2008
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Translocation of a tRNA with an extended anticodon through the ribosome
Overview
Coordinated translocation of the tRNA-mRNA complex by the ribosome occurs, in a precise, stepwise movement corresponding to a distance of three, nucleotides along the mRNA. Frameshift suppressor tRNAs generally contain, an extra nucleotide in the anticodon loop and they subvert the normal, mechanisms used by the ribosome for frame maintenance. The mechanism by, which suppressor tRNAs traverse the ribosome during translocation is, poorly understood. Here, we demonstrate translocation of a tRNA by four, nucleotides from the A site to the P site, and from the P site to the E, site. We show that translocation of a punctuated mRNA is possible with an, extra, unpaired nucleotide between codons. Interestingly, the NMR, structure of the four nucleotide anticodon stem-loop reveals a, conformation different from the canonical tRNA structure. Flexibility, within the loop may allow conformational adjustment upon A site binding, and for interacting with the four nucleotide codon in order to shift the, mRNA reading frame.
About this Structure
2GV3 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Translocation of a tRNA with an extended anticodon through the ribosome., Phelps SS, Gaudin C, Yoshizawa S, Benitez C, Fourmy D, Joseph S, J Mol Biol. 2006 Jul 14;360(3):610-22. Epub 2006 May 22. PMID:16787653
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