2gzs

From Proteopedia

---

Revision as of 18:11, 29 January 2008

proteopedia linkproteopedia link

spinqualitylabelsall models 2gzs, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Enterobactin Hydolase IroE Complex with DFP

Overview

The proliferation of many pathogenic bacteria is limited by the scarcity, of soluble iron in their environment. Many of these bacteria scavenge iron, by synthesizing and exporting small molecule siderophores that chelate, iron. Iron-bound siderophores are subsequently imported for metabolic, processing. Three related serine hydrolases have been characterized, biochemically in this pathway: Fes, IroD, and IroE. Here, we report the, crystal structure of IroE from uropathogenic Escherichia coli CFT073. The, native structure and a complex with diisopropyl fluorophosphonate (DFP, a, potent serine hydrolase inhibitor) were determined at 2.3 and 1.4 A, resolution, respectively. IroE has the typical alpha/beta-hydrolase fold, with an atypical catalytic dyad composed of Ser 189 and His 287. Mutation, of either residue was detrimental to catalysis. In addition, rather than, the typical oxyanion hole composed of backbone amides, IroE employs the, atypical guanidinium moiety of Arg 130. Asp 90 anchors Arg 130 in the, active site, and mutation of either residue was likewise detrimental to, catalysis. We also compare the structure of IroE to the structure of Fes, from Shigella flexneri (PDB entry 2B20). Both enzymes have similar active, sites, but Fes has an additional amino-terminal lid domain. These lid, domains are proposed to confer specificity to these related hydrolases.

About this Structure

2GZS is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.

Reference

Structural characterization of enterobactin hydrolase IroE., Larsen NA, Lin H, Wei R, Fischbach MA, Walsh CT, Biochemistry. 2006 Aug 29;45(34):10184-90. PMID:16922493

Page seeded by OCA on Tue Jan 29 20:11:16 2008