Group:MUZIC:Enigma Family
From Proteopedia
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The member proteins have all been located to the mammalian muscle cells, some specific to the heart and skeletal muscle Z-disk. They interact via their PDZ domains with motor protein components of the Z-disk and also recruit signalling molecules via their LIM domains or internal motifs, for example ''ZM motif'' (ZASP-like motif which is sandwiched between the PDZ- and LIM-domains in ZASP)<ref>doi:10.1161/CIRCRESAHA.110.225615</ref>. These interactions via their PDZ- and LIM-domains have been suggested to be important for targeting/sustaining interacting protein complexes within the sarcomere for a physiologically functional muscle. | The member proteins have all been located to the mammalian muscle cells, some specific to the heart and skeletal muscle Z-disk. They interact via their PDZ domains with motor protein components of the Z-disk and also recruit signalling molecules via their LIM domains or internal motifs, for example ''ZM motif'' (ZASP-like motif which is sandwiched between the PDZ- and LIM-domains in ZASP)<ref>doi:10.1161/CIRCRESAHA.110.225615</ref>. These interactions via their PDZ- and LIM-domains have been suggested to be important for targeting/sustaining interacting protein complexes within the sarcomere for a physiologically functional muscle. | ||
| - | ==''''' | + | =='''Enigma'''==<StructureSection load='PDLIM7.pdb' size='300' side='right' caption='Crystal structure of PDZ domain of Enigma/PDLIM7 protein (PDB entry: [[2Q3G]])' scene='User:Adekunle_Onipe/workbench/Enigma_Family/Pdz_enigma/2' |
| - | Enigma protein, alternatively referred to as PDLIM7 (PDZ and LIM domain protein 7) is the first and representative member of the Enigma subfamily. Initially characterized in human as ~49.85 kDa protein with an N-terminal PDZ domain and three C-terminal LIM domains <ref>PMID:7929196</ref>, <ref>PMID:10359609</ref>. Five alternatively spliced isoforms are presently identified [http://www.uniprot.org/uniprot/Q9NR12#section_features] | + | >Enigma protein, alternatively referred to as PDLIM7 (PDZ and LIM domain protein 7) is the first and representative member of the Enigma subfamily. Initially characterized in human as ~49.85 kDa protein with an N-terminal PDZ domain and three C-terminal LIM domains <ref>PMID:7929196</ref>, <ref>PMID:10359609</ref>. Five alternatively spliced isoforms are presently identified [http://www.uniprot.org/uniprot/Q9NR12#section_features] |
| + | '''''Sequence Annotation''''' | ||
| - | + | Assembly of the PDZ- and LIM-domains of the first Enigma isoform identified and characterized ('''457 amino acids''') is as illustrated below [Q9NR12] [http://www.uniprot.org/uniprot/Q9NR12#section_features] <ref>PMID:7929196</ref>: | |
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| - | Assembly of the PDZ- and LIM-domains of the first | + | |
[[Image:Enigma.png]] | [[Image:Enigma.png]] | ||
| - | '''Structure''' | + | '''''Structure''''' |
| - | X-ray crystal structure of | + | Shown to the right, the X-ray crystal structure of Enigma PDZ domain has recently been solved to 1.11Å. This revealed a canonical PDZ-domain fold containing six strands and 2 helices. |
| - | '''Function and Interactions''' | + | '''''Function and Interactions''''' |
| - | '''Pathology''' | + | '''''Pathology''''' |
=='''''Enigma Homologue (ENH) protein'''''== | =='''''Enigma Homologue (ENH) protein'''''== | ||
Revision as of 10:16, 7 July 2011
Enigma family: PDZ- and LIM-domain protein of the cytoskeleton
Three member proteins have extensively been described and characterized within this subfamily: Enigma protein, Enigma Homologue (ENH) protein and ZASP/Cypher/Oracle ('ZASP'[1] being the human orthologue of 'Cypher'[2] which is found in mouse and also identified by independent researchers who named it 'Oracle'[3]).
Didactically, protein members of the enigma subfamily typically possess within their structure: (1) an N-terminal PDZ domain (a domain which is named after the first three proteins where it was initially characterized i.e. PSD 95, Disc large protein and Zonula Occludens 1), and (2) three C-terminal LIM domain (a domain which is named after the first three proteins where it was characterized i.e. Lin-11, Isl1 and Mec-3)[4].
The member proteins have all been located to the mammalian muscle cells, some specific to the heart and skeletal muscle Z-disk. They interact via their PDZ domains with motor protein components of the Z-disk and also recruit signalling molecules via their LIM domains or internal motifs, for example ZM motif (ZASP-like motif which is sandwiched between the PDZ- and LIM-domains in ZASP)[5]. These interactions via their PDZ- and LIM-domains have been suggested to be important for targeting/sustaining interacting protein complexes within the sarcomere for a physiologically functional muscle.
==Enigma==
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