2h3g
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(New page: 200px<br /><applet load="2h3g" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h3g, resolution 2.000Å" /> '''Structure of the Ty...)
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Revision as of 18:13, 29 January 2008
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Structure of the Type III Pantothenate Kinase (CoaX) from Bacillus Anthracis
Overview
Coenzyme A (CoASH) is the major low-molecular weight thiol in, Staphylococcus aureus and a number of other bacteria; the crystal, structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which, maintains the reduced intracellular state of CoASH, has recently been, reported [Mallett, T.C., Wallen, J.R., Karplus, P.A., Sakai, H., Tsukihara, T., and Claiborne, A. (2006) Biochemistry 45, 11278-89]. In, this report we demonstrate that CoASH is the major thiol in Bacillus, anthracis; a bioinformatics analysis indicates that three of the four, proteins responsible for the conversion of pantothenate (Pan) to CoASH in, Escherichia coli are conserved in B. anthracis. In contrast, a novel type, III pantothenate kinase (PanK) catalyzes the first committed step in the, biosynthetic pathway in B. anthracis; unlike the E. coli type I PanK, this, enzyme is not subject to feedback inhibition by CoASH. The crystal, structure of B. anthracis PanK (BaPanK), solved using multiwavelength, anomalous dispersion data and refined at a resolution of 2.0 A, demonstrates that BaPanK is a new member of the Acetate and Sugar, Kinase/Hsc70/Actin (ASKHA) superfamily. The Pan and ATP substrates have, been modeled into the active-site cleft; in addition to providing a clear, rationale for the absence of CoASH inhibition, analysis of the Pan-binding, pocket has led to the development of two new structure-based motifs (the, PAN and INTERFACE motifs). Our analyses also suggest that the type III, PanK in the spore-forming B. anthracis plays an essential role in the, novel thiol/disulfide redox biology of this category A biodefense, pathogen.
About this Structure
2H3G is a Single protein structure of sequence from Bacillus anthracis with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the Type III Pantothenate Kinase from Bacillus anthracis at 2.0 A Resolution: Implications for Coenzyme A-Dependent Redox Biology(,)., Nicely NI, Parsonage D, Paige C, Newton GL, Fahey RC, Leonardi R, Jackowski S, Mallett TC, Claiborne A, Biochemistry. 2007 Mar 20;46(11):3234-3245. Epub 2007 Feb 27. PMID:17323930
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