2hlr
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(New page: 200px<br /><applet load="2hlr" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hlr, resolution 1.200Å" /> '''Crystal Structure o...)
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Revision as of 18:23, 29 January 2008
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Crystal Structure of the Extracellular Domain of the Type II BMP Receptor
Overview
BMPRII is a type II TGF-beta serine threonine kinase receptor which is, integral to the bone morphogenetic protein (BMP) signalling pathway. It is, known to bind BMP and growth differentiation factor (GDF) ligands, and has, overlapping ligand specificity with the activin type II receptor, ActRII., In contrast to activin and TGF-beta type ligands, BMPs bind to type II, receptors with lower affinity than type I receptors. Crystals of the, BMPRII ectodomain were grown in two different forms, both of which, diffracted to high resolution. The tetragonal form exhibited some, disorder, whereas the entire polypeptide was seen in the orthorhombic, form. The two structures retain the basic three-finger toxin fold of other, TGF-beta receptor ectodomains, and share the main hydrophobic patch used, by ActRII to bind various ligands. However, they present different, conformations of the A-loop at the periphery of the proposed, ligand-binding interface, in conjunction with rearrangement of a disulfide, bridge within the loop. This particular disulfide (Cys94-Cys117) is only, present in BMPRII and activin receptors, suggesting that it is important, for their likely shared mode of binding. Evidence is presented that the, two crystal forms represent ligand-bound and free conformations of BMPRII., Comparison with the solved structure of ActRII bound to BMP2 suggests that, His87, unique amongst TGF-beta receptors, may play a key role in ligand, recognition.
About this Structure
2HLR is a Single protein structure of sequence from Ovis aries. Active as Receptor protein serine/threonine kinase, with EC number 2.7.11.30 Full crystallographic information is available from OCA.
Reference
High resolution structures of the bone morphogenetic protein type II receptor in two crystal forms: implications for ligand binding., Mace PD, Cutfield JF, Cutfield SM, Biochem Biophys Res Commun. 2006 Dec 29;351(4):831-8. Epub 2006 Nov 10. PMID:17094948
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