2hmt
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(New page: 200px<br /><applet load="2hmt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hmt, resolution 2.200Å" /> '''Diamond-shaped octa...)
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Revision as of 18:24, 29 January 2008
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Diamond-shaped octameric ring structure of an RCK domain with NADH bound
Overview
The KtrAB ion transporter is a complex of the KtrB membrane protein and, KtrA, an RCK domain. RCK domains regulate eukaryotic and prokaryotic, membrane proteins involved in K(+) transport. Conflicting functional, models have proposed two different oligomeric arrangements for RCK, domains, tetramer versus octamer. Our results for the KtrAB RCK domain, clearly show an octamer in solution and in the crystal. We determined the, structure of this protein in three different octameric ring conformations, that resemble the RCK-domain octamer observed in the MthK potassium, channel but show striking differences in size and symmetry. We present, experimental evidence for the association between one RCK octameric ring, and two KtrB membrane proteins. These results provide insights into the, quaternary organization of the KtrAB transporter and its mechanism of, activation and show that the RCK-domain octameric ring model is generally, applicable to other ion-transport systems.
About this Structure
2HMT is a Single protein structure of sequence from Bacillus subtilis with as ligand. Full crystallographic information is available from OCA.
Reference
The RCK domain of the KtrAB K+ transporter: multiple conformations of an octameric ring., Albright RA, Ibar JL, Kim CU, Gruner SM, Morais-Cabral JH, Cell. 2006 Sep 22;126(6):1147-59. PMID:16990138
Page seeded by OCA on Tue Jan 29 20:24:23 2008
Categories: Bacillus subtilis | Single protein | Albright, R.A. | Morais-Cabral, J.H. | NAI | Ion transporter | Ktn | Ktr | Ktra | Ktrab | Membrane protein | Rck | Symporter
