Group:USC-LCHS
From Proteopedia
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==Example: Hoogsteen base pairs modulate shape of p53-DNA binding site== | ==Example: Hoogsteen base pairs modulate shape of p53-DNA binding site== | ||
<StructureSection load='3kz8' size='500' side='left' caption='(PDB entry [[3kz8]])' scene=''> | <StructureSection load='3kz8' size='500' side='left' caption='(PDB entry [[3kz8]])' scene=''> | ||
| - | + | ===Publication Abstract from PubMed=== | |
| + | p53 binds as a tetramer to DNA targets consisting of two decameric half-sites separated by a variable spacer. Here we present high-resolution crystal structures of complexes between p53 core-domain tetramers and DNA targets consisting of contiguous half-sites. In contrast to previously reported p53-DNA complexes that show standard Watson-Crick base pairs, the newly reported structures show noncanonical Hoogsteen base-pairing geometry at the central A-T doublet of each half-site. Structural and computational analyses show that the Hoogsteen geometry distinctly modulates the B-DNA helix in terms of local shape and electrostatic potential, which, together with the contiguous DNA configuration, results in enhanced protein-DNA and protein-protein interactions compared to noncontiguous half-sites. Our results suggest a mechanism relating spacer length to protein-DNA binding affinity. Our findings also expand the current understanding of protein-DNA recognition and establish the structural and chemical properties of Hoogsteen base pairs as the basis for a novel mode of sequence readout. | ||
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| + | ''Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs., Kitayner M, Rozenberg H, Rohs R, Suad O, Rabinovich D, Honig B, Shakked Z, Nat Struct Mol Biol. 2010 Apr;17(4):423-9. Epub 2010 Apr 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/20364130 20364130]'' | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. | ||
</StructureSection> | </StructureSection> | ||
==References== | ==References== | ||
<references/> | <references/> | ||
Revision as of 00:32, 20 July 2011
Contents |
USC-LCHS: University of Southern California - La Cañada High School
Welcome, scientists, to your home page for the joint structural biology initiative between the University of Southern California (USC) and La Cañada High School (LCHS), hosted on the Proteopedia web resource. The goal is... . You'll notice that this is not a regular webpage. If you scroll down a bit, you'll find that there are interactive 3D structures of proteins and nucleic acids on this page. You can even trigger animations by clicking on green hyperlinks in the text when you find them--these green hyperlinks will change the orientation and representation of the 3D structure in order to illustrate a point made in the text. The best part is that it is easy to create your own page with an interactive 3D structure and green links, and that is what you will be doing to present your findings! Feel free to also explore other pages in Proteopedia that are not related to USC-LCHS. Proteopedia is a collaborative 3D encyclopedia of proteins and other biomolecules, and you will find many interesting proteins described in interactive detail.
Example: DNA shape is the molecular basis for Hox specificity
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Example: Hoogsteen base pairs modulate shape of p53-DNA binding site
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