2i7z
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(New page: 200px<br /><applet load="2i7z" size="350" color="white" frame="true" align="right" spinBox="true" caption="2i7z" /> '''GAAA tetraloop receptor complex with associa...)
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Revision as of 18:36, 29 January 2008
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GAAA tetraloop receptor complex with associated manganese ions.
Overview
Metal ions are critical for the proper folding of RNA, and the GAAA, tetraloop-receptor is necessary for the optimal folding and function of, many RNAs. We have used NMR to investigate the role of metal ions in the, structure of the tetraloop-receptor in solution. The NMR data indicate, native tertiary structure is formed under a wide range of ionic, conditions. The lack of conformational adaptation in response to very, different ionic conditions argues against a structural role for divalent, ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic, relaxation enhancement induced by manganese ions were used to determine, the NMR structures of the tetraloop receptor in association with metal, ions, providing the first atomic-level view of these interactions in the, solution state. Five manganese and two cobalt hexammine ions could be, localized to the RNA surface. The locations of the associated metal ions, are similar, but not identical to, those of previously determined crystal, structures. The sites of association are in general agreement with, nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA, tertiary structure.
About this Structure
2I7Z is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:17119098
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