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2j23
From Proteopedia
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(New page: 200px<br /><applet load="2j23" size="350" color="white" frame="true" align="right" spinBox="true" caption="2j23, resolution 1.41Å" /> '''CROSS-REACTIVITY AND...)
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Revision as of 18:50, 29 January 2008
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CROSS-REACTIVITY AND CRYSTAL STRUCTURE OF MALASSEZIA SYMPODIALIS THIOREDOXIN (MALA S 13), A MEMBER OF A NEW PAN-ALLERGEN FAMILY
Overview
We have identified thioredoxins (Trx) of Malassezia sympodialis, a yeast, involved in the pathogenesis of atopic eczema, and of Aspergillus, fumigatus, a fungus involved in pulmonary complications, as novel, IgE-binding proteins. We show that these Trx, including the human enzyme, represent cross-reactive structures recognized by serum IgE from, individuals sensitized to M. sympodialis Trx. Moreover, all three proteins, were able to elicit immediate-type allergic skin reactions in sensitized, individuals, indicating a humoral immune response based on molecular, mimicry. To analyze structural elements involved in these reactions, the, three-dimensional structure of M. sympodialis Trx (Mala s 13) has been, determined at 1.4-A resolution by x-ray diffraction analysis. The, structure was solved by molecular replacement and refined to a, crystallographic R factor of 14.0% and a free R factor of 16.8% and shows, the typical Trx fold. Mala s 13 shares 45% sequence identity with human, Trx and superposition of the solved Mala s 13 structure with those of, human Trx reveals a high similarity with a root mean square deviation of, 1.11 A for all Calpha atoms. In a detailed analysis of the molecular, surface in combination with sequence alignment, we identified conserved, solvent-exposed amino acids scattered over the surface in both structures, which cluster to patches, thus forming putative conformational B cell, epitopes potentially involved in IgE-mediated cross- and autoreactivity.
About this Structure
2J23 is a Single protein structure of sequence from Malassezia sympodialis. Full crystallographic information is available from OCA.
Reference
Cross-reactivity and 1.4-A crystal structure of Malassezia sympodialis thioredoxin (Mala s 13), a member of a new pan-allergen family., Limacher A, Glaser AG, Meier C, Schmid-Grendelmeier P, Zeller S, Scapozza L, Crameri R, J Immunol. 2007 Jan 1;178(1):389-96. PMID:17182577
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