This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1oi8
From Proteopedia
(New page: 200px<br /> <applet load="1oi8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oi8, resolution 2.10Å" /> '''5'-NUCLEOTIDASE (E....) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1OI8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MN, SO4 and CO3 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OI8 OCA]]. | + | 1OI8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MN, SO4 and CO3 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OI8 OCA]]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: udp-sugar hydrolase]] | [[Category: udp-sugar hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:18:41 2007'' |
Revision as of 10:13, 30 October 2007
|
5'-NUCLEOTIDASE (E. COLI) WITH AN ENGINEERED DISULFIDE BRIDGE (P90C, L424C)
Overview
Engineering disulfide bridges is a common technique to lock a protein, movement in a defined conformational state. We have designed two double, mutants of Escherichia coli 5'-nucleotidase to trap the enzyme in both an, open (S228C, P513C) and a closed (P90C, L424C) conformation by the, formation of disulfide bridges. The mutant proteins have been expressed, purified, and crystallized, to structurally characterize the designed, variants. The S228C, P513C is a double mutant crystallized in two, different crystal forms with three independent conformers, which differ, from each other by a rotation of up to 12 degrees of the C-terminal domain, with respect to the N-terminal domain. This finding, as well as an, analysis of the domain motion in the crystal, indicates that the enzyme, still ... [(full description)]
About this Structure
1OI8 is a [Single protein] structure of sequence from [Escherichia coli] with MN, SO4 and CO3 as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Trapping a 96 degrees domain rotation in two distinct conformations by engineered disulfide bridges., Schultz-Heienbrok R, Maier T, Strater N, Protein Sci. 2004 Jul;13(7):1811-22. PMID:15215524
Page seeded by OCA on Tue Oct 30 12:18:41 2007
