1oid
From Proteopedia
(New page: 200px<br /> <applet load="1oid" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oid, resolution 2.10Å" /> '''5'-NUCLEOTIDASE (E....) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OID is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OID OCA]]. | + | 1OID is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OID OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: udp-sugar hydrolase]] | [[Category: udp-sugar hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:18:53 2007'' |
Revision as of 10:14, 30 October 2007
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5'-NUCLEOTIDASE (E. COLI) WITH AN ENGINEERED DISULFIDE BRIDGE (S228C, P513C)
Overview
Engineering disulfide bridges is a common technique to lock a protein, movement in a defined conformational state. We have designed two double, mutants of Escherichia coli 5'-nucleotidase to trap the enzyme in both an, open (S228C, P513C) and a closed (P90C, L424C) conformation by the, formation of disulfide bridges. The mutant proteins have been expressed, purified, and crystallized, to structurally characterize the designed, variants. The S228C, P513C is a double mutant crystallized in two, different crystal forms with three independent conformers, which differ, from each other by a rotation of up to 12 degrees of the C-terminal domain, with respect to the N-terminal domain. This finding, as well as an, analysis of the domain motion in the crystal, indicates that the enzyme, still ... [(full description)]
About this Structure
1OID is a [Single protein] structure of sequence from [Escherichia coli] with NI as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Trapping a 96 degrees domain rotation in two distinct conformations by engineered disulfide bridges., Schultz-Heienbrok R, Maier T, Strater N, Protein Sci. 2004 Jul;13(7):1811-22. PMID:15215524
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