1oim
From Proteopedia
(New page: 200px<br /> <applet load="1oim" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oim, resolution 2.15Å" /> '''FAMILY 1 B-GLUCOSID...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OIM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with NOJ as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OIM OCA]]. | + | 1OIM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with NOJ as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OIM OCA]]. |
==Reference== | ==Reference== | ||
Iminosugar glycosidase inhibitors: structural and thermodynamic dissection of the binding of isofagomine and 1-deoxynojirimycin to beta-glucosidases., Zechel DL, Boraston AB, Gloster T, Boraston CM, Macdonald JM, Tilbrook DM, Stick RV, Davies GJ, J Am Chem Soc. 2003 Nov 26;125(47):14313-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14624580 14624580] | Iminosugar glycosidase inhibitors: structural and thermodynamic dissection of the binding of isofagomine and 1-deoxynojirimycin to beta-glucosidases., Zechel DL, Boraston AB, Gloster T, Boraston CM, Macdonald JM, Tilbrook DM, Stick RV, Davies GJ, J Am Chem Soc. 2003 Nov 26;125(47):14313-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14624580 14624580] | ||
| + | [[Category: Beta-glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:19:15 2007'' |
Revision as of 10:14, 30 October 2007
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FAMILY 1 B-GLUCOSIDASE FROM THERMOTOGA MARITIMA
Overview
The design and synthesis of transition-state mimics reflects the growing, need both to understand enzymatic catalysis and to influence strategies, for therapeutic intervention. Iminosugars are among the most potent, inhibitors of glycosidases. Here, the binding of 1-deoxynojirimycin and, (+)-isofagomine to the "family GH-1" beta-glucosidase of Thermotoga, maritima is investigated by kinetic analysis, isothermal titration, calorimetry, and X-ray crystallography. The binding of both of these, iminosugar inhibitors is driven by a large and favorable enthalpy. The, greater inhibitory power of isofagomine, relative to 1-deoxynojirimycin, however, resides in its significantly more favorable entropy; indeed the, differing thermodynamic signatures of these inhibitors are further, highlighted by ... [(full description)]
About this Structure
1OIM is a [Single protein] structure of sequence from [Thermotoga maritima] with NOJ as [ligand]. Active as [Beta-glucosidase], with EC number [3.2.1.21]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Iminosugar glycosidase inhibitors: structural and thermodynamic dissection of the binding of isofagomine and 1-deoxynojirimycin to beta-glucosidases., Zechel DL, Boraston AB, Gloster T, Boraston CM, Macdonald JM, Tilbrook DM, Stick RV, Davies GJ, J Am Chem Soc. 2003 Nov 26;125(47):14313-23. PMID:14624580
Page seeded by OCA on Tue Oct 30 12:19:15 2007
