2o1i

From Proteopedia

Revision as of 19:03, 29 January 2008

RH(BPY)2CHRYSI complexed to mismatched DNA

Overview

We report the 1.1-A resolution crystal structure of a bulky rhodium, complex bound to two different DNA sites, mismatched and matched in the, oligonucleotide 5'-(dCGGAAATTCCCG)2-3'. At the AC mismatch site, the, structure reveals ligand insertion from the minor groove with ejection of, both mismatched bases and elucidates how destabilized mispairs in DNA may, be recognized. This unique binding mode contrasts with major groove, intercalation, observed at a matched site, where doubling of the base pair, rise accommodates stacking of the intercalator. Mass spectral analysis, reveals different photocleavage products associated with the two binding, modes in the crystal, with only products characteristic of mismatch, binding in solution. This structure, illustrating two clearly distinct, binding modes for a molecule with DNA, provides a rationale for the, interrogation and detection of mismatches.

About this Structure

2O1I is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Insights into finding a mismatch through the structure of a mispaired DNA bound by a rhodium intercalator., Pierre VC, Kaiser JT, Barton JK, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):429-34. Epub 2006 Dec 28. PMID:17194756

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