310d

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spinqualitylabelsall models 310d, resolution 1.300Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF 2'-O-ME(CGCGCG)2: AN RNA DUPLEX AT 1.3 A RESOLUTION. HYDRATION PATTERN OF 2'-O-METHYLATED RNA

Overview

The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been, determined using synchrotron radiation at near-atomic resolution (1.30 A), the highest resolution to date in the RNA field. The crystal structure is, a half-turn A-type helix with some helical parameters deviating from, canonical A-RNA, such as low base pair rise, elevated helical twist and, inclination angles. In CG steps, inter-strand guanines are parallel while, cytosines are not parallel. In steps GC this motif is reversed. This type, of regularity is not seen in other RNA crystal structures. The structure, includes 44 water molecules and two hydrated Mg2+ions one of which lies, exactly on the crystallographic 2-fold axis. There are distinct patterns, of hydration in the major and the minor grooves. The major groove is, stabilised by water clusters consisting of fused five- and six-membered, rings. Minor groove contains only a single row of water molecules; each, water bridges either two self-parallel cytosines or two self-parallel, guanines by a pair of hydrogen bonds. The structure provides the first, view of the hydration scheme of 2'-O-methylated RNA duplex.

About this Structure

310D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA., Adamiak DA, Milecki J, Popenda M, Adamiak RW, Dauter Z, Rypniewski WR, Nucleic Acids Res. 1997 Nov 15;25(22):4599-607. PMID:9358171

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