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333d
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(New page: 200px<br /><applet load="333d" size="350" color="white" frame="true" align="right" spinBox="true" caption="333d, resolution 2.520Å" /> '''THE CRYSTAL STRUCTU...)
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Revision as of 19:35, 29 January 2008
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THE CRYSTAL STRUCTURE OF AN RNA OLIGOMER INCORPORATING TANDEM ADENOSINE-INOSINE MISMATCHES
Overview
The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has, been refined to 2.5 A. It shows a symmetric internal loop of two, non-Watson-Crick base pairs which form in the middle of the duplex. The, tandem A-I/I-A pairs are related by a crystallographic two-fold axis. Both, A(anti)-I(anti) mismatches are in a head-to-head conformation forming, hydrogen bonds using the Watson-Crick positions. The octamer duplexes, stack above one another in the cell forming a pseudo-infinite helix, throughout the crystal. A hydrated calcium ion bridges between the, 3'-terminal of one molecule and the backbone of another. The tandem A-I, mismatches are incorporated with only minor distortion to the backbone., This is in contrast to the large helical perturbations often produced by, sheared G-A pairs in RNA oligonucleotides.
About this Structure
333D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of an RNA oligomer incorporating tandem adenosine-inosine mismatches., Carter RJ, Baeyens KJ, SantaLucia J, Turner DH, Holbrook SR, Nucleic Acids Res. 1997 Oct 15;25(20):4117-22. PMID:9321667
Page seeded by OCA on Tue Jan 29 21:35:40 2008
