335d

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Revision as of 19:35, 29 January 2008

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spinqualitylabelsall models 335d, resolution 2.400Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

THE CRYSTAL STRUCTURE OF THE DNA DECAMER D(GGCAATTGCG) CONTAINS BOTH MAJOR AND MINOR GROOVE BINDING G.(G.C) TRIPLETS

Overview

The crystal structure of the decamer d(GGCAATTGCG) has been determined at, 2.4 A resolution. The central eight bases of each DNA single-strand base, pair with a self-complementary strand to form an octamer B-DNA duplex., These duplexes lie end-to-end within the unit cell. The terminal 5'-G and, G-3' bases of each decamer strand are unpaired, and interact with the, neighbouring duplexes via interactions within both the major and minor, groove. This results in base triplets of the type G-(G.C) and G*(G.C), with the third guanine base binding to a Watson-Crick G.C base pair from, the major groove and the minor groove, respectively. The triplet, interaction of the type G-(G.C) involves Hoogsteen hydrogen-bonding, interactions between the two guanine bases. The minor- and major-groove, base triplet interactions which exist within this structure act to, stabilize the d(GCAATTGC)2 B-DNA octamer duplex.

About this Structure

335D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Structure of the DNA decamer d(GGCAATTGCG) contains both major- and minor-groove binding G.(G.C) base triplets., Nunn CM, Neidle S, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):577-83. PMID:9761852

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