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359d

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(New page: 200px<br /><applet load="359d" size="350" color="white" frame="true" align="right" spinBox="true" caption="359d, resolution 2.900&Aring;" /> '''INHIBITION OF THE H...)
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Revision as of 19:36, 29 January 2008

Image:359d.jpg

359d, resolution 2.900Å

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INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)

Overview

Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by, competing with a single magnesium(II) ion. X-ray crystallography revealed, that the Tb(III) ion binds to a site adjacent to an essential guanosine in, the catalytic core of the ribozyme, approximately 10 angstroms from the, cleavage site. Synthetic modifications near this binding site yielded an, RNA substrate that was resistant to Tb(III) binding and capable of being, cleaved, even in the presence of up to 20 micromolar Tb(III). It is, suggested that the magnesium(II) ion thought to bind at this site may act, as a switch, affecting the conformational changes required to achieve the, transition state.

About this Structure

359D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:9417029

Page seeded by OCA on Tue Jan 29 21:36:35 2008

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